Sains Malaysiana 54(7)(2025): 1677-1685

http://doi.org/10.17576/jsm-2025-5407-03

 

Study on the Mechanism of miRNA9718 Inhibition of the STAT3 Pathway Exacerbating Candida albicans-Induced Intestinal Epithelial Cell Damage

(Kajian tentang Mekanisme miRNA9718 Perencatan Laluan STAT3 Memburukkan Kerosakan Sel Epitelium Usus Aruhan Candida albicans)

 

JINYING ZHAO, LIPING YIN, LINXIN JIANG, SIJIA SHEN, JIANGUO TANG & XUE YIN*

 

Department of Trauma-Emergency & Critical Care Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai, China

 

Diserahkan: 6 September 2024/Diterima: 27 Disember 2024

 

Abstract

Candida albicans is a commensal yeast pathogen that frequently colonizes the human gastrointestinal (GI) tract, and this capacity to efficiently invade allows it access to deeper tissues leading to disease. Probing this resistance is necessary to develop effective treatment strategies and requires further understanding of how intestinal epithelial cells resist invasion by C. albicans. In this study, we performed miRNA transcriptomic analysis combined with experiments to screen differentially expressed miRNAs under C. albicans exposure in intestinal epithelial cells. We also identified a subset of miRNAs that were notably deregulated by C. albicans invasion, largely within the context of targeting STAT3. Taken together, we provide new insights on how intestinal epithelial cells counteract C. albicans invasion. The STAT3 pathway and its regulation of the dysregulated miRNAs are evidenced by our studies. These results further the progress in intestinal host-pathogen interaction and has implications for possible C. albicans directed therapeutics approaches.

Keywords: Candida albicans; intestinal epithelial cells; mechanism of resistance; miRNA omics analysis; STAT3 pathway

 

Abstrak

Candida albicans adalah patogen kulat oportunistik yang biasa dikenali kerana keupayaannya untuk menyerang dan menyebabkan jangkitan gastrointestinal. Memahami mekanisme apabila sel epitel usus menolak serangan oleh C. albicans adalah penting untuk membangunkan strategi rawatan yang berkesan. Untuk mengenal pasti miRNA yang berubah ekspresinya sebagai tindak balas kepada pendedahan C. albicans dalam sel epitel usus, kami melakukan analisis transkriptomik miRNA dan mengesahkan hasil tersebut melalui uji kaji. Analisis kami menunjukkan satu kumpulan miRNA yang mengalami disregulasi secara signifikan semasa serangan C. albicans, terutamanya yang menjejaskan laluan STAT3. Kajian kami memberikan pandangan baharu tentang mekanisme apabila sel epitel usus menolak serangan oleh C. albicans. Kami tunjukkan keterlibatan laluan STAT3 dan peranan kawalan miRNA yang disregulasi dalam proses ini. Penemuan ini menyumbang kepada pemahaman kita tentang interaksi hos-patogen dalam saluran gastrointestinal dan mungkin mempunyai implikasi dalam pembangunan pendekatan terapeutik baharu yang menumpukan kepada jangkitan C. albicans.

Kata kunci: Analisis omik miRNA; Candida albicans; laluan STAT3; mekanisme perlindungan; sel epitel usus

 

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*Pengarang untuk surat-menyurat; email: 13524177588@163.com